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Discovery HS PEG HPLC Columns 120Å;
3µm, 5µm and 10µm particle sizes available |
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A unique polyethylene glycol reversed phase column specifically developed for pharmaceutical
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analysis and purification
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Unique selectivity and faster separations of polar compounds compared to C18 phases
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Outstanding performance for phenolic compounds offers unique retention and selectivity and
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faster analysis compared to C18
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Scalable from analytical to prep
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Highly stable to ensure excellent run-to-run and lot-to-lot reproducibility and long column life
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| UNIQUE SELECTIVITY AND FASTER SEPARATION OF POLAR COMPOUNDS |
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Discovery HS PEG delivers unique retention, selectivity and faster separations of polar compounds when compared to C18 phases.
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Columns: Conventional C18 phase and Discovery HS PEG, 15cm x 4.6mm, 5µm; Mobile Phase: 85% 10mM ammonium acetate, pH 6.8 : 15% MeCN; Flow Rate: 1.0mL/min; Temp: 20°C; Detection: UV/Photodiode Array; Injection: 10µL (50µg/mL for each analyte)
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Conventional C18 Phase
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1. Uracil
2. Benzamide
3. Acetanilide
4. Phenol
5. N-Methylaniline
6. Nitrobenzene
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Discovery HS PEG
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1. Uracil
2. Benzamide
3. Acetanilide
4. Phenol
5. N-Methylaniline
6. Nitrobenzene
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OUTSTANDING RESOLUTION OF PHENOLIC
COMPOUNDS ON DISCOVERY HS PEG |
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Columns: Conventional C18 phase and Discovery HS PEG, 15cm x 4.6mm, 5µm; Mobile Phase: 85% 10mM ammonium acetate, pH 6.8 : 15% MeCN; Flow Rate: 1.0mL/min; Temp: 20°C; Detection: UV/Photodiode Array; Injection: 10µL (50µg/mL for each analyte)
1. Uracil
2. Phloroglucinicol
3. Pyrogallol
4. Resorcinol
5. Benzamide
6. Catechol
7. Phenol
8. Nitrobenzene
9. Phenetole
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Especially note the improved retention of Phloroglucinicol (Peak 2) and Phenetole (Peak 9) on the Discovery HS PEG phase.
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| LOT TO LOT REPRODUCIBILITY OF DISCOVERY HS PEG |
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Column: Discovery HS PEG, 15cm x 4.6mm, 5µm; Mobile Phase: 45% 10mm potassium phosphate, pH 7 : 55% MeOH; Flow Rate: 1mL/min; Temp: 35°C; Detection: 254nm; Injection: 5µL
1. Uracil (8µg/mL)
2. Pyridine (20µg/mL)
3. Phenol (300µg/mL)
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| Ordering Information |
| 3µm Discovery HS PEG HPLC Columns |
| ID(mm) |
Length(cm) |
Cat. No. |
| 2.1 |
5 |
567400-U |
| 2.1 |
10 |
567402-U |
| 2.1 |
15 |
567403-U |
| 4.0 |
5 |
567430-U |
| 4.0 |
10 |
567431-U |
| 4.0 |
15 |
567432-U |
| 4.6 |
5 |
567404-U |
| 4.6 |
10 |
567406-U |
| 4.6 |
15 |
567407-U |
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| 5µm Discovery HS PEG HPLC Columns |
| ID(mm) |
Length(cm) |
Cat. No. |
| 2.1 |
5 |
567408-U |
| 2.1 |
10 |
567410-U |
| 2.1 |
15 |
567411-U |
| 2.1 |
25 |
567412-U |
| 4.0 |
5 |
567433-U |
| 4.0 |
10 |
567434-U |
| 4.0 |
15 |
567435-U |
| 4.0 |
25 |
567436-U |
| 4.6 |
5 |
567413-U |
| 4.6 |
10 |
567415-U |
| 4.6 |
15 |
567416-U |
| 4.6 |
25 |
567417-U |
| 10.0 |
5 |
567418-U |
| 10.0 |
10 |
567437-U |
| 10.0 |
15 |
567419-U |
| 10.0 |
25 |
567420-U |
| 21.2 |
5 |
567421-U |
| 21.2 |
10 |
567439-U |
| 21.2 |
15 |
567422-U |
| 21.2 |
25 |
567423-U |
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| 10µm Discovery HS PEG HPLC Columns |
| ID(mm) |
Length(cm) |
Cat. No. |
| 10.0 |
5 |
567424-U |
| 10.0 |
10 |
567438-U |
| 10.0 |
15 |
567425-U |
| 10.0 |
25 |
567426-U |
| 21.2 |
5 |
567427-U |
| 21.2 |
10 |
567440-U |
| 21.2 |
15 |
567428-U |
| 21.2 |
25 |
567429-U |
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| 2cm Supelguard Cartridges with Discovery HS PEG Packings |
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Description |
2.1mm ID |
4.0mm ID  |
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3µm Particle Size |
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567470-U |
567472-U |
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Kit  |
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567471-U |
567473-U |
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5µm Particle Size |
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567474-U |
567476-U |
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Kit |
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567475-U |
567477-U |
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For 4.0mm ID or 4.6mm ID analytical columns |
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Kit includes cartridge, stand-alone holder, tubing, 2 nuts and ferrules. |
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| 1cm Supelguard Cartridges with Discovery HS PEG Packings |
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Description |
10mm ID |
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5µm Particle Size |
567478-U |
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10µm Particle Size |
567480-U |
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 To download a printable copy of this information in PDF format, click here. |
| TRADEMARKS |
| Discovery and Supelguard are trademarks of Sigma-Aldrich Co. |
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