LC-MS CHROMASOLV®
- Solvents for LC-MS Analysis
LC-NMR CHROMASOLV®
- Solvents for LC-NMR Analysis
Fluka Solvents for HPLC
In liquid chromatography the quality and reproducibility of the separation depends not only on the properties of the stationary phase, but decisively on the quality of the solvents used.
Fluka has been optimized all HPLC solvents for liquid chromatography applications. HPLC solvents are lot-tested and precisely controlled to meet the high quality requirements of successful HPLC separations:
- Low UV-absorbance, assuring optimum sensitivity
- Guaranteed suitability for fluorescence detection
- Controlled low content of non-volatile components like water, free acid and free alkali, assuring long pump-seal life
- Low particulates, pre-filtration is not necessary
- High assay, assuring high purity
Gradient grade HPLC solvents are specified with a gradient elution test to illustrate the interference-free baselines. Both UV absorbance and fluorescence signals are monitored.
All HPLC solvents are delivered with an expiration date.
Product Range
| Catalog No. |
Product |
Purity |
Package Size |
| M6502 |
Acetone * |
for HPLC; >99.8% (GC) |
1 l, 2.5 l |
| 00692 |
Acetonitrile * |
for HPLC; >99.8% (GC) |
500 ml, 1 l, 2.5 l |
| 00683 |
Acetonitrile |
for HPLC; gradient grade+ suitable for PAK-analysis; >99.9% (GC) |
1 l, 2.5 l |
| 12549 |
Benzene |
for HPLC; >99.5% (GC) (dried material) |
1 l, 2.5 l |
| 19417 |
1-Butanol * |
for HPLC; >99.8% (GC) |
1 l, 2.5 l |
| 20398 |
2-Butoxyethanol |
for HPLC - GC; >99.8% (GC) |
500 ml, 1 l |
| 20247 |
tert-Butyl methyl ether * |
for HPLC; >99.8% (GC) |
1 l, 2.5 l |
| 87036 |
Carbon tetrachloride |
for HPLC; >99.8% (GC) |
1 l |
| 19748 |
1-Chlorobutane * |
for HPLC; >99.8% (GC) |
1 l |
| 25685 |
Chloroform * |
for HPLC; >99.8% (GC); stab. with ~1% ethanol |
1 l, 2.5 l |
| 59379 |
2-Chloropropane |
for HPLC; >99.5% (GC) |
500 ml, 1 l |
| 28931 |
Cyclohexane * |
for HPLC; >99.5% (GC) |
1 l, 2.5 l |
| 29681 |
Cyclopentane |
for HPLC; >75% (GC) |
1 l, 2.5 l |
| 03522 |
1,2-Dichloroethane |
for HPLC; >99.8% (GC) |
500 ml, 1 l |
| 66746 |
Dichloromethane * |
for HPLC; >99.8% (GC) |
1 l, 2.5 l |
| 31675 |
Diethyl ether, stab. |
for HPLC; >99.8% (GC); stab. with 2,6-di-tert-butyl-p-cresol |
1 l, 2.5 l |
| 38569 |
1,2-Dimethoxyethane |
for HPLC; >99.5% (GC) |
500 ml, 1 l |
| 38842 |
N,N-Dimethylacetamide |
for HPLC; >99.8% (GC) |
1 l, 2.5 l |
| 42505 |
Dioxane * |
for HPLC; >99.8% (GC) |
500 ml, 1 l, 2.5 l |
| 02854 |
Ethanol |
for HPLC; gradient grade >99.8% (v/v) |
1 l, 2.5 l |
| 02855 |
Ethanol |
for HPLC; >99.8% (v/v) |
1 l, 2.5 l |
| 45763 |
Ethyl acetate * |
for HPLC; >99.8% (GC) |
1 l, 2.5 l |
| 51742 |
Heptane * |
for HPLC; >99.5% (GC) |
500 ml, 1 l, 2.5 l |
| 52762 |
Hexane * |
for HPLC; >95% (GC) |
1 l, 2.5 l |
| 59042 |
Isooctane * |
for HPLC; >99.5% (GC) |
1 l, 2.5 l |
| 65548 |
Methanol |
for HPLC; gradient grade >99.8% (GC) |
1 l, 2.5 l |
| 65541 |
Methanol * |
for HPLC; >99.8% (GC) |
1 l, 2.5 l |
| 45999 |
Methyl acetate |
for HPLC; >99.8% (GC) |
250 ml, 1 l, 2.5 l |
| 73479 |
Nitromethane |
for HPLC; >97.0% (GC) |
250 ml, 1 l |
| 76875 |
Pentane * |
for HPLC; >98.0% (GC) |
500 ml, 1 l |
| 82091 |
1-Propanol * |
for HPLC; >99.8% (GC) |
1 l, 2.5 l |
| 59298 |
2-Propanol |
for HPLC; gradient grade >99.8% (GC) |
1 l, 2.5 l |
| 59307 |
2-Propanol * |
for HPLC; >99.8% (GC) |
1 l, 2.5 l |
| 86968 |
Tetrachloroethylene |
for HPLC; gradient grade >99.5% (GC) |
1 l, 2.5 l |
| 87367 |
Tetrahydrofuran * |
for HPLC; >99.8% (GC) |
500 ml, 1 l, 2.5 l |
| 89683 |
Toluene * |
for HPLC; gradient grade >99.5% (GC) |
1 l, 2.5 l |
| 95270 |
Water |
for gradient elution |
1 l, 5 l, 10 l |
| 95304 |
Water |
for HPLC; filtered through a 0.2 mm membrane filter |
1 l, 2.5 l |
* becomes deleted with replacement, please click here for details
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Concept of High-Purity Solvents for HPLC
In high performance liquid chromatography the quality and reproducibility of the separation depends not only on the properties of the stationary phase, but decisively on the quality of the solvents used. The solvent line CHROMASOLV® offers guaranteed quality specially tailored to chromatographic requirements, in conjunction with batch consistency. Please find below the different product groups with description and detailed product lists.
| G CHROMASOLV® solvents for gradient elution |
CHROMASOLV® solvents for HPLC |
E CHROMASOLV® solvents for HPLC |
| The G CHROMASOLV® qualities were developed for sensitive gradient elutions at short wavelengths and with very high requirements with regard to UV-transmittance and fluorescence detection. |
The CHROMASOLV® solvents are characterized by high UV-transmittance, consistently gradient testing for interfering peaks and base line drift, guaranteed suitability for fluorescence detection, low non-volatile components, free acid and free alkali and an exactly defined low water content. |
This quality is suitable for measurements in the low wavelength range and has also been tested for suitability for gradient applications and for fluorescence detection. |
| P CHROMASOLV® solvents for preparative LC |
R CHROMASOLV® solvents for routine measurements in LC |
The specifications of the
P CHROMASOLV® qualities are tailored fully to the requirements of preparative liquid chromatography. In particular this means low contents of non-volatile impurities and of free alkali. |
This quality is available for routine analysis in which chromatography is not carried out with gradient elution at short wavelengths. It is suitable for isocratic analysis, for example at 210 nm or with gradients at 254 nm. |
Example Specification
| |
Acetonitrile
G CHROMASOLV® Prod.No. 34998 |
Acetonitrile CHROMASOLV® Prod.No. 34851 |
Acetonitrile
E CHROMASOLV® Prod.No. 34888 |
| assay (GC) |
min. 99.9 % |
min. 99.9 % |
min. 99.9 % |
| non-volatile matter |
max. 0.0002 % |
max. 0.0005 % |
max. 0.0005 % |
| water (Karl Fischer) |
max. 0.01 % |
max. 0.02 % |
max. 0.02 % |
| free acid (as CH3COOH) |
max. 0.001 % |
max. 0.001 % |
max. 0.002 % |
| free alkali (as NH3) |
max. 0.0001 % |
max. 0.0002 % |
max. 0.0002 % |
| transmittance at 195 nm |
min. 80 % |
min. 75 % |
min. 65 % |
| transmittance at 200 nm |
min. 95 % |
min. 93 % |
min. 88 % |
| transmittance at 210 nm |
- |
- |
min. 93 % |
| transmittance from 228 nm |
min. 99 % |
- |
- |
| transmittance from 230 nm |
- |
min. 99 % |
- |
| transmittance from 235 nm |
- |
- |
min. 99 % |
| HPLC-gradient at 210 nm |
max. 1 mAU |
max. 3 mAU |
- |
| baseline drift at 210 nm |
max. 12 mAU |
max. 15 mAU |
- |
| HPLC-gradient at 254 nm |
max. 0.2 mAU |
max. 0.5 mAU |
- |
| fluorescence at 254 nm |
max. 0.5 ppb |
max. 1 ppb |
max. 1 ppb |
| fluorescence at 365 nm |
max. 0.5 ppb |
max. 0.5 ppb |
max. 0.5 ppb |
| |
Acetonitrile
P CHROMASOLV® Prod.No. 34989 |
Acetonitrile
R CHROMASOLV® Prod.No. 34881 |
| assay (GC) |
min. 99.8 % |
min. 99.8 % |
| non-volatile matter |
max. 0.0001 % |
max. 0.0005 % |
| water (Karl Fischer) |
max. 0.01 % |
max. 0.01 % |
| free acid (as CH3COOH) |
- |
max. 0.001 % |
| free alkali (as NH3) |
max. 0.0005 % |
- |
| transmittance at 200 nm |
- |
min. 50 % |
| transmittance at 220 nm |
min. 50 % |
min. 90 % |
| transmittance from 240 nm |
min. 99 % |
min. 99 % |
Product Range
|
G CHROMASOLV®
|
| 34998 |
Acetonitrile G CHROMASOLV® super gradient grade |
1; 2.5; 7; 18; 45; 200 L |
| 34963 |
Ethanol G CHROMASOLV® |
1; 2.5 L |
| 34964 |
Ethanol G CHROMASOLV® (for export) |
1; 2.5 L |
| 34885 |
Methanol G CHROMASOLV® |
1; 2.5; 7; 18; 45; 200 L |
| 34959 |
2-Propanol G CHROMASOLV® |
1; 2.5 L |
| 34877 |
Water G CHROMASOLV® |
1; 2.5 L |
|
|
| 34850 |
Aceton CHROMASOLV® |
1; 2.5; 45 L |
| 34851 |
Acetonitrile CHROMASOLV® gradient grade |
1; 2.5; 7; 18; 45; 200 L |
| 34867 |
1-Butanol CHROMASOLV® |
2.5 L |
| 34861 |
Butanone CHROMASOLV® |
1; 2.5 L |
| 34875 |
tert.-Butylmethylether CHROMASOLV® |
1; 2.5; 7 L |
| 34958 |
1-Chlorobutane CHROMASOLV® |
1 L |
| 34855 |
Cyclohexane CHROMASOLV® |
1; 2.5; 7 L |
| 34872 |
1,2-Dichloroethane CHROMASOLV® |
1 L |
| 34856 |
Dichloromethane CHROMASOLV® |
1; 2.5; 7 L |
| 34889 |
Dimethoxymethane CHROMASOLV® |
2.5; 7 L |
| 34869 |
Dimethylsulfoxide CHROMASOLV® |
1; 2.5 L |
| 34857 |
1,4-Dioxane CHROMASOLV® |
1; 2.5 L |
| 34858 |
Ethylacetate CHROMASOLV® |
1; 2.5 L |
| 34852 |
ethanol CHROMASOLV® |
1; 2.5; 7 L |
| 34870 |
ethanol CHROMASOLV® (for export) |
1; 2.5 L |
| 34873 |
n-Heptane CHROMASOLV® |
1; 2.5; 7; 45 L |
| 34994 |
Iso-Hexane CHROMASOLV® |
1; 2.5 L |
| 34859 |
n-Hexane CHROMASOLV® |
1; 2.5; 7; 45 L |
| 34860 |
methanol CHROMASOLV® |
1; 2.5; 7; 18; 45; 200 L |
| 34862 |
Iso-Octane CHROMASOLV® |
2.5 L |
| 34956 |
n-Pentane CHROMASOLV® |
1; 2.5 L |
| 34871 |
1-Propanol CHROMASOLV® |
1; 2.5 L |
| 34863 |
2-Propanol CHROMASOLV® |
1; 2.5; 7; 45 L |
| 34864 |
Carbon tetrachloride CHROMASOLV® |
1 L |
| 34865 |
Tetrahydrofuran CHROMASOLV® |
1; 2.5; 7; 45 L |
| 34866 |
Toluene CHROMASOLV® |
1; 2.5; 18; 45 L |
| 34854 |
Trichloromethane CHROMASOLV® |
1; 2.5; 45 L |
| 34874 |
1,1,2-Trichlorotrifluoroethane CHROMASOLV® |
1; 2.5 L |
|
|
| 34888 |
Acetonitrile E CHROMASOLV®, far UV |
1; 2.5; 18 L |
|
|
| 34989 |
Acetonitrile P CHROMASOLV® |
2.5; 18; 45; 185 L |
| 34973 |
tert.-Butylmethylether P CHROMASOLV® |
18; 45; 200 L |
| 34977 |
Dichloromethane P CHROMASOLV® |
45; 200 L |
| 34979 |
Ethylacetate P CHROMASOLV® |
2.5; 18; 45; 185 L |
| 34981 |
n-Heptane P CHROMASOLV® |
2.5; 18; 45 L |
| 34969 |
iso-Hexane P CHROMASOLV® |
2.5; 45; 200 L |
| 34993 |
n-Hexane P CHROMASOLV® |
2.5; 200 L |
| 34983 |
Methanol P CHROMASOLV® |
2.5; 18; 45; 185 L |
| 34991 |
1-Propanol P CHROMASOLV® |
2.5; 200 L |
| 34985 |
2-Propanol P CHROMASOLV® |
18; 45; 185 L |
| 34987 |
Toluene P CHROMASOLV® |
18; 45; 200 L |
| 34975 |
Trichloromethane P CHROMASOLV® |
18; 45; 200 L |
|
|
| 34881 |
Acetonitrile R CHROMASOLV®, far UV |
1; 2.5; 7; 18; 45; 200 L |
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Concept of LC-MS CHROMASOLV® Solvents for LC-MS Analysis
IMPROVE STABILITY OF LC-MS BASELINES BY USING HIGH-QUALITY SOLVENTS
Using LC-MS CHROMASOLV® eliminates impurities and the need to lot-select your solvents.
LC-MS is fast becoming the dominant analytical tool for researchers in a wide range of industries and application areas. LC-MS combines the advantages of a chromatographic separation with mass detection by MS. Low detection limits and qualified analysis of molecular structures brought this application a new and growing popularity especially in the field of biotechnology and within the R&D departments of the pharmaceutical industry.
Besides the column and instrument, the solvent can seriously affect the performance of an LC-MS system. Impurities arising from the solvent can mask low levels of target ions, or give false peaks, resulting in faulty interpretation of spectra. Sigma-Aldrich's Riedel-de Haën brand of LC-MS CHROMASOLV solvents is manufactured specifically to meet the needs of modern LC-MS instruments because they are designed to have low contents (max. 100 ppb) of alkaline impurities, such as calcium, magnesium, potassium and sodium, which can form clusters with the analyte, especially when using electro spray ionization. All LC-MS CHROMASOLV solvents undergo a battery of specific UV-spectroscopic quality control tests to guarantee the traditional Riedel-de Haën CHROMASOLV specifications and to ensure the reproducibility. They are also checked for LC-MS use by a special test and for particles, which could clog up Nano-LC inlet filters.
For more details see our latest researches: pdf 370 KB.
| 39253 |
Water LC-MS CHROMASOLV® |
1 L |
Specifications/Product Range
| LC-MS solvent |
Water |
Acetonitrile |
Methanol |
2-Propanol |
Ethylacetate |
| product number |
39253 |
34967 |
34966 |
34965 |
34972 |
| pack sizes |
1 L |
1 L / 2.5 L |
1 L / 2.5 L |
1 L / 2.5 L |
1 L / 2.5 L |
| assay (GC) |
- |
min. 99.9 % |
min. 99.9 % |
min. 99.9 % |
min. 99.7 % |
| fluorescence at 254 nm |
max. 1 ppb |
max. 0.5 ppb |
max. 1 ppb |
max.1 ppb |
- |
| fluorescence at 365 nm |
max. 1 ppb |
max. 0.5 ppb |
max. 1 ppb |
max. 1ppb |
- |
| Chlorid (Cl) |
max. 0.000001% |
- |
- |
- |
- |
| Fluorid (F) |
max. 0.000001% |
- |
- |
- |
- |
| Nitrate (NO3) |
max. 0.00001% |
- |
- |
- |
- |
| Sulfate (SO4) |
max. 0.00001% |
- |
- |
- |
- |
| free acid |
- |
max. 0.001 % |
max. 0.001 % |
max. 0.001 % |
- |
| free alkali (as NH3) |
- |
max. 0.0002 % |
max. 0.0005 % |
max. 0.0005 % |
max. 0.0005 % |
| non-volatile matter |
max. 0.001 % |
max. 0.0002 % |
max. 0.0005 % |
max. 0.0005 % |
max. 0.0005 % |
| water (Karl Fischer) |
- |
max. 0.01 % |
max. 0.02 % |
max. 0.05 % |
max. 0.03 % |
| transmittance at 200 nm |
min. 95% |
min. 95% |
- |
- |
- |
| transmittance at 230 nm |
min. 99% |
min. 99% |
min. 75% |
min. 75% |
- |
| transmittance at 260 m |
- |
- |
min. 98% |
min. 98% |
min. 50% |
| HPLC gradient (254 nm) |
max. 1 mAU |
max. 0.2 mAU |
max. 2 mAU |
max. 2 mAU |
- |
| Silver (Ag) |
max. 0.1 ppm |
max. 0.1 ppm |
max. 0.1 ppm |
max. 0.1 ppm |
- |
| Aluminum (Al) |
max. 0.5 ppm |
max. 0.5 ppm |
max. 0.5 ppm |
max. 0.5 ppm |
- |
| Barium (Ba) |
max. 0.1 ppm |
max. 0.1 ppm |
max. 0.1 ppm |
max. 0.1 ppm |
- |
| Calcium (Ca) |
max. 0.1 ppm |
max. 0.1 ppm |
max. 0.1 ppm |
max. 0.1 ppm |
max. 0.1 ppm |
| Cadmium (Cd) |
max. 0.05 ppm |
max. 0.05 ppm |
max. 0.05 ppm |
max. 0.05 ppm |
- |
| Cobalt (Co) |
max. 0.02 ppm |
max. 0.02 ppm |
max. 0.02 ppm |
max. 0.02 ppm |
- |
| Chromium (Cr) |
max. 0.02 ppm |
max. 0.02 ppm |
max. 0.02 ppm |
max. 0.02 ppm |
- |
| Copper (Cu) |
max. 0.02 ppm |
max. 0.02 ppm |
max. 0.01 ppm |
max. 0.02 ppm |
- |
| Iron (Fe) |
max. 0.1 ppm |
max. 0.1 ppm |
max. 0.1 ppm |
max. 0.1 ppm |
- |
| Potassium (K) |
max. 0.1 ppm |
max. 0.1 ppm |
max. 0.1 ppm |
max. 0.1 ppm |
max. 0.1 ppm |
| Magnesium (Mg) |
max. 0.1 ppm |
max. 0.1 ppm |
max. 0.1 ppm |
max. 0.1 ppm |
max. 0.1 ppm |
| Manganese (Mn) |
max. 0.02 ppm |
max. 0.02 ppm |
max. 0.01 ppm |
max. 0.02 ppm |
- |
| Sodium (Na) |
max. 0.1 ppm |
max. 0.1 ppm |
max. 0.1 ppm |
max. 0.1 ppm |
max. 0.1 ppm |
| Nickel (Ni) |
max. 0.02 ppm |
max. 0.02 ppm |
max. 0.02 ppm |
max. 0.02 ppm |
- |
| Lead (Pb) |
max. 0.1 ppm |
max. 0.1 ppm |
max. 0.02 ppm |
max. 0.1 ppm |
- |
| Tin (Sn) |
max. 0.1 ppm |
max. 0.1 ppm |
max. 0.1 ppm |
max. 0.1 ppm |
- |
| Zinc (Zn) |
max. 0.1 ppm |
max. 0.1 ppm |
max. 0.1 ppm |
max. 0.1 ppm |
- |
| particle test |
+ |
+ |
+ |
- |
- |
| LC-MS suitability test |
+ |
+ |
+ |
+ |
+ |
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LC-MS CHROMASOLV® - Solvent Blends
LC-MS blends
LC-MS allows the detection and quantification of many analytes. The minimisation of artefacts requires very well specified solvents spiked with ultra pure salts and acids. These additives are used to improve the chromatographic peak shape and to optimise ionisation in the MS interface. The most commonly used solvents in LC-MS techniques are acetonitrile, methanol and water. Additives can include trifluoracetic acid, formic acid, acetic acid and ammonium acetate.
For more details see our latest Labinfo: pdf 104 KB
| Catalog No. |
Maktx |
Package Size |
Specification |
| 34976 |
ACETONITRILE WITH 0.1 VOL.% TRIFLUORACETIC ACID LC-MS CHROMASOLV® |
2.5L amber bottle |
LC gradient testing in UV and MS, metals impurities (Na < 2ppm, K, Mg, Ca
< 0.5ppm), UV-Absorption, additive content: 0.093-0.107 (TFA, FA, AA: v/v, Ammoniumacetat: g/v) Solvents content: (GC): > 99.0% (exeption 34669 Acetonitrile with 0.1% ammonium acetate:
> 98%)
|
| 34678 |
ACETONITRILE WITH 0.1% ACETIC ACID LC-MS CHROMASOLV® |
2.5L amber bottle |
| 34669 |
ACETONITRILE WITH 0.1% AMMONIUM ACETATE LC-MS CHROMASOLV® |
2.5L amber bottle |
| 34668 |
ACETONITRILE WITH 0.1% FORMIC ACID LC-MS CHROMASOLV® |
2.5L amber bottle |
| 34672 |
METHANOL WITH 0.1% ACETIC ACID LC-MS CHROMASOLV® |
2.5L amber bottle |
| 34670 |
METHANOL WITH 0.1% AMMONIUM ACETATE LC-MS CHROMASOLV® |
2.5L amber bottle |
| 34974 |
METHANOL WITH 0.1% TRIFLUOROACETIC ACID LC-MS CHROMASOLV® |
2.5L amber bottle |
| 34978 |
WATER WITH 0.1 VOL.% TRIFLUORACETIC ACID LC-MS CHROMASOLV® |
2.5L amber bottle |
LC gradient testing in UV and MS, metals impurities (Na < 2ppm, K, Mg, Ca
< 0.5ppm), UV-Absorption, additive content: 0.093-0.107 (TFA, FA, AA: v/v, Ammonium acetate: g/v), pH: effective
+/- 0.1 |
| 34675 |
WATER WITH 0.1% ACETIC ACID LC-MS CHROMASOLV® |
2.5L amber bottle |
| 34674 |
WATER WITH 0.1% AMMONIUM ACETATE LC-MS CHROMASOLV® |
2.5L amber bottle |
| 34673 |
WATER WITH 0.1% FORMIC ACID LC-MS CHROMASOLV® |
2.5L amber bottle |
| 34689 |
WATER/2-PROPANOL LC-MS CHROMASOL LC-MS CHROMASOLV® |
1L white glass bottle |
rinsing agent for
LC-MS |
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LC-NMR CHROMASOLV® Solvents for LC-NMR Analysis
Although an efficient separation method is available by HPLC, no structure determination is possible when detecting separated compounds by UV-detector. Thus NMR spectroscopy is a better universial detection method. A combination of both methods was evident and LC-NMR was born.
Solvents that meet the high requirements of HPLC and proton NMR are needed. But the high throughput of solvents in HPLC does not allow the usage of expensive deuterated solvents as usually required for NMR. HPLC Acetonitrile is often contaminated with impurities containing protons such as propionitrile which is no problem in UV but NMR detection. Therefore a dramatic decrease of proton containing contaminants for LC-NMR solvents is essential.
Acetonitrile LC-NMR CHROMASOLV® (34955) from Riedel-de Haën is the solvent of choice. It was developed to obtain the superior specifications required for LC-NMR. There are no further signals in 1H-NMR that exceed the size of methyl signals (more than 5% at 3.78 ppm NMR of 0.0006% trimethoxybenzene) at typical LC-NMR conditions.
| |
Acetonitrile/ Prod.No. 34955 / 0.5 L / 1 L |
| assay (GC) |
min. 99.9 % |
| non-volatile matter |
max. 0.0002 % |
| water (Karl Fischer) |
max. 0.01 % |
| free acid (as CH3COOH) |
max. 0.001 % |
| free alkali (as NH3) |
max. 0.0001 % |
| propionitrile (GC) |
max. 0.0002 % |
For additional information, please contact your local Sigma-Aldrich office.
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