Ion Pair Reagents

The use of ion-pairing reagents as mobile phase additives allows the separation of ionic and highly polar substances on reversed phase HPLC columns. The purity of mobile phase additives is of utmost importance to their successful application.

Fluka offers an outstanding range of tailor-made reagents for anionic (quaternary ammonium and phosphonium salts) and cationic (alkanesulfonates) determination (see Table 1). All mobile phase additives are subject to rigorous testing with special emphasis on the requirements of modern reversed phase HPLC:

• Filter test
• UV-absorption test
• Test for the absence of redox traces with a cyclic voltammogram
• HPLC gradient grade test

Table 1. Tailor-made reagents for anionic and cationic determination

Catalog No.	Product Name	CAS No.	Description	Package Size	Add to Cart
19022	1-Butanesulfonic acid, sodium salt	2386-54-1	puriss. p.a. for ion pair chromatography, >99.0% (T)	2.5 g, 10 g, 50 g
19029	1-Butanesulfonic acid, sodium salt, conc.	2386-54-1	puriss. p.a. for ion pair chromatography	6 ampoules
30518	Decamethonium bromide	541-22-0	puriss. p.a. for ion pair chromatography, >99.0% (AT)	5 g, 10 g
30631	1-Decanesulfonic acid, sodium salt	13419-61-9	puriss. p.a. for ion pair chromatography, ampoule	2.5 g, 10 g, 50 g
44123	1-Dodecanesulfonic acid, sodium salt	2386-53-0	puriss. p.a. for ion pair chromatography, >99.0% (T)	2.5 g, 10 g, 50 g
44239	Dodecyl trimethyl ammonium bromide	1119-94-4	puriss. p.a. for ion pair chromatography, >99.0% (AT)	5 g, 10 g
44243	Dodecyl trimethyl ammonium hydrogensulfate	103999-25-3	puriss. p.a. for ion pair chromatography, >99.0% (T)	2.5 g, 10 g
02374	1,2-Ethane disulfonic acid Disodium salt	5325-43-9	puriss. p.a. for ion pair chromatography, >99.0% (T)	5 g, 25 g
51832	1-Heptane sulfonic acid, sodium salt monohyd., for ion pair chrom.	22767-50-6	puriss. p.a. for ion pair chromatography, >99. 0% (T)	2.5 g, 10 g, 50 g
51834	1-Heptane sulfonic acid, sodium salt, conc.	22767-50-6	puriss. p.a. for ion pair chromatography	6 ampoules
52263	11-Hexadecane sulfonic acid, sodium salt	15015-81-3	puriss. p.a. for ion pair chromatography, >99.0% (T)	2.5 g, 10 g
52367	Hexadecyl trimethyl ammonium bromide	57-09-0	puriss. p.a. for ion pair chromatography, >99.0% (AT)	2.5 g, 10 g, 50 g
52363	Hexadecyl trimethyl ammonium dihydrogen phosph., conc.	111412-68-1	puriss. p.a. for ion pair chromatography	6 ampoules
52371	Hexadecyl trimethyl ammonium hydrogensulfate	68214-07-3	puriss. p.a. for ion pair chromatography, >99.0% (T)	5 g
52382	Hexadecyl trimethyl amm. Hydroxide, conc.	505-86-2	puriss. p.a. for ion pair chromatography	6 ampoules
52862	1-Hexane sulfonic acid sodium salt, monohydrate	2832-45-3	puriss. p.a. for ion pair chromatography, >99.0% ((T)	2.5 g, 10 g, 50 g
52864	1-Hexane sulfonic acid sodium salt conc.	2832-45-3	puriss. p.a. for ion pair chromatography	6 ampoules
70289	Naphthalene- 2-sulfonic acid (beta) sodium salt	532-02-5	puriss. p.a. for ion pair chromatography, >99.0% (HPLC)	10 g, 50 g
74316	1-Nonane sulfonic acid sodium salt	35192-74-6	puriss. p.a. for ion pair chromatography, >99.0% (T)	2.5 g, 10 g
74845	1,8-Octanediyl- bis(tributyl phosphonium) bis(hydrogen sulf.)	532-02-5	puriss. p.a. for ion pair chromatography, >99.0% (T)	10 g
74826	1,8-Octanediyl- bis(tributyl phosphonium) dibromide	131526-14-2	puriss. p.a. for ion pair chromatography, >99.0% (AT )	10 g
74882	1-Octane sulfonic acid sodium salt monohydrate	5324-84-5	puriss. p.a. for ion pair chromatography, >99.0% (T)	2.5 g, 10 g, 50 g
74886	1-Octane sulfonic acid sodium salt conc.	5324-84-5	puriss. p.a. for ion pair chromatography	6 ampoules
75073	Octyl sulfate sodium salt	142-31-4	puriss. p.a. for ion pair chromatography, >99.0% (T)	2.5 g, 10 g
76952	1-Pentane sulfonic acid sodium salt monohydrate	22767-49-3	puriss. p.a. for ion pair chromatography, >99.0% (T)	2.5 g, 10 g, 50 g
76954	1-Pentane sulfonic acid sodium salt, conc.	22767-49-3	puriss. p.a. for ion pair chromatography, ampoule	6 ampoules
81806	1-Propane sulfonic acid sodium salt	14533-63-2	puriss. p.a. for ion pair chromatography, >99.0% (T)	2.5 g, 10 g, 50 g
81808	2-Propane sulfonic acid sodium salt monohydrate	5399-58-6	puriss. p.a. for ion pair chromatography, >99.0% (T )	10 g
71443	Sodium decyl sulfate	142-87-0	puriss. p.a. for ion pair chromatography, >99.0% (T)	2.5 g, 10 g
71726	Sodium dodecyl sulfate	151-21-3	puriss. p.a. for ion pair chromatography, >99.0% (GC)	5 g, 10 g, 50 g
71735	Sodium dodecyl sulfate concentrate	151-21-3	puriss. p.a. for ion pair chromatography	6 ampoules
74734	Sodium 1-octadecane sulfonate	13893-34-0	puriss. p.a. for ion pair chromatography, >99.0% (T)	2.5 g
86857	Tetrabutyl ammonium bromide	1643-19-2	puriss. p.a. for ion pair chromatography, >99.0% (AT)	6 ampoules
86846	Tetrabutyl ammonium bromide, conc.	1643-19-2	puriss. p.a. for ion pair chromatography	6 ampoules
86852	Tetrabutyl ammonium chloride	1112-67-0	puriss. p.a. for ion pair chromatography, >99.0% (AT)	2.5 g, 10 g, 50 g
86862	Tetrabutyl ammonium chloride, conc.	1112-67-0	puriss. p.a. for ion pair chromatography	6 ampoules
86899	Tetrabutyl ammonium dihydrogen phosphate, conc.	5574-97-0	puriss. p.a. for ion pair chromatography	6 ampoules
86853	Tetrabutyl ammonium hydrogen sulfate	32503-27-8	puriss. p.a. for ion pair chromatography, >99.0% (T)	2.5 g, 10 g, 50 g
86847	Tetrabutyl ammonium hydrogen sulfate, conc.	32503-27-8	puriss. p.a. for ion pair chromatography	6 ampoules
86851	Tetrabutyl ammonium hydroxide, conc.	2052-49-5	puriss. p.a. for ion pair chromatography	6 ampoules
86903	Tetrabutylammonium iodide	311-28-4	puriss. p.a. for ion pair chromatography, >99.0% (AT)	2.5 g, 10 g
86915	Tetrabutyl phosphonium bromide	3115-68-2	puriss. p.a. for ion pair chromatography, >99.0 % (AT)	10 g
86925	Tetrabutyl phosphonium hydrogen sulfate	108203-01-6	puriss. p.a. for ion pair chromatography; >99.0% (T)	10 g
87191	1-Tetradecane sulfonic acid, sodium salt	6994-45-2	puriss. p.a. for ion pair chromatography, >99.0% (T)	2.5 g, 10 g
87208	Tetradecyl trimethyl ammonium bromide	1119-97-7	puriss. p.a. for ion pair chromatography, >99.0% (AT)	5 g, 10 g
87215	Tetradecyl trimethyl ammonium hydrogensulfate	104903-23-3	puriss. p.a. for ion pair chromatography, >99.0% (T)	2.5 g, 10 g
86608	Tetraethyl ammonium bromide	71-91-0	puriss. p.a. for ion pair chromatography, >99.0% (AT)	10 g
86626	Tetraethyl ammonium hydrogen sulfate	16873-13-5	puriss. p.a. for ion pair chromatography, >99.0% (T)	5 g, 10 g, 50 g
86635	Tetraethyl ammonium hydroxide, conc.	77-98-5	puriss. p.a. for ion pair chromatography	6 ampoules
87296	Tetraheptyl ammonium bromide	4368-51-8	puriss. p.a. for ion pair chromatography, >99.0% (AT)	5 g, 10 g, 50 g
87297	Tetrahexylammonium bromide	4328-13-6	puriss. p.a. for ion pair chromatography, >99.0% (AT)	10 g
87313	Tetrahexyl ammonium dihydrogen phosphate, conc.	87700-05-8	puriss. p.a. for ion pair chromatography	6 ampoules
87299	Tetrahexyl ammonium hydrogen sulfate	32503-34-7	puriss. p.a. for ion pair chromatography, >99.0% (T)	2.5 g, 5 g, 25 g
87578	Tetrakis (decyl) ammonium bromide	14937-42-9	puriss. p.a. for ion pair chromatography, >99.0% (AT)	5 g, 10 g, 50 g
87708	Tetramethyl ammonium bromide	64-20-0	puriss. p.a. for ion pair chromatography, >99.0% (AT)	10 g
87724	Tetramethyl ammonium hydrogen sulfate	80526-82-5	puriss. p.a. for ion pair chromatography, >99.0% (T)	5 g, 10 g, 50 g
87727	Tetramethyl ammonium hydrogen sulfate, conc.	80526-82-5	puriss. p.a. for ion pair chromatography	6 ampoules
87728	Tetramethyl ammonium hydroxide, conc.	75-59-2	puriss. p.a. for ion pair chromatography	6 ampoules
02799	Tetramethyl ammonium sulfate	14190-16-0	puriss. p.a. for ion pair chromatography, >99.0% (T)	2.5 g, 10 g, 50 g
87996	Tetraoctyl ammonium bromide	14866-33-2	puriss. p.a. for ion pair chromatography, >99.0% (AT)	10 g
87997	Tetrapentyl ammonium bromide	866-97-7	puriss. p.a. for ion pair chromatography, >99.0% (AT)	10 g
88103	Tetrapropyl ammonium bromide	1941-30-6	puriss. p.a. for ion pair chromatography, >99.0% (AT)	10 g
88106	Tetrapropyl ammonium hydrogen sulfate	56211-70-2	puriss. p.a. for ion pair chromatography, >99.0% (T)	5 g, 10 g
88109	Tetrapropyl ammonium hydroxide, conc.	4499-86-9	puriss. p.a. for ion pair chromatography	6 ampoules
94133	1-Undecane sulfonic acid, sodium salt	5838-34-6	puriss. p.a. for ion pair chromatography, >99.0% (T)	2.5 g, 10 g

For example, tetrabutylammonium hydogensulfate shows excellent transparency down to 200 nm, even at high concentrations. In addition, the reagents are tested for the absence of insoluble materials and non-absorbing impurities like redox traces, which may interfere with the sample. A final check is performed with an application test, using a very rough gradient (see Figure A, B and C).